[Activation mechanism of calcium-activated neutral protease].
نویسنده
چکیده
The activation mechanism through limited autolysis of a calcium-activated neutral protease (CANP) with a high sensitivity to calcium ions (pCANP) was analyzed. The rate of autolysis was dependent on pCANP concentration. The reaction was inhibited by high concentrations of digestible substrates but not by a nondigestible substrate. Incubation of pCANP inactivated by Nethylmaleimide with a small amount of activated pCANP caused the degradation of the former in a manner similar to the autolysis of native pCANP. Immobilized pCANP bound to an anti-pCANP immunoglobulin G column autolyzed on addition of calcium ions. These results show that activation of pCANP through limited autolysis involves both intramolecular and intermolecular reactions.
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ورودعنوان ژورنال:
- Seikagaku. The Journal of Japanese Biochemical Society
دوره 54 11 شماره
صفحات -
تاریخ انتشار 1982